Figure 7. Co-localisation of fTRG tagged proteins with endogenous proteins.
Antibody stainings of the adult thorax from fTRG or wild-type lines with anti-GFP antibody (green or white in the single colour images) and antibodies against various fly proteins (red). (A-D) Co-localisation of LanA-GFP with anti-Laminin antibody stain around the midgut (A), of Par6-GFP with anti-Par6 at the apical side of the proventriculus epithelium (B) and of Mlp84B-GFP as well as Mhc-GFP with anti-Mlp84B and anti-Mhc antibody stain in leg muscles, respectively (C, D). (E-H) Adult thoraces from wild-type flies show very similar patterns with the respective antibodies. (I-L) Adult IFMs showing the co-localisation of Fln-GFP with anti-Fln antibody staining (I), Unc-89/Obscurin-GFP with anti-Obscurin antibody staining (J), Dlg1-GFP with anti-Dlg1 antibody staining (K) and Mhc-GFP with anti Mhc antibody staining (L). (M-P) The same antibodies result in very similar patterns in wild-type IFMs apart from the a sharp versus a diffuse Mhc pattern comparing wild-type to Mhc-GFP flies (L, P). (Q) Western blots loaded with total protein extract from wild-type, Mlp84B-GFP, Fln-GFP and Dlg1-GFP adult males probed with anti-V5 (included in the GFP tag) anti-Mlp84B, anti-Fln and anti-Dlg1 antibodies. Note the about 40 kDa size shift of the tagged proteins in the respective lanes (marked with green arrow heads) versus the untagged protein band (black arrow heads).