Video 6. Z-projection of a two-photon Video of an about 14 hr APF pupa expressing βTub60D-GFP.
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DOI: 10.7554/eLife.12068.030
A stack was acquired every 20 min for 25 hr. Note the high expression of βTub60D-GFP in fusing myoblasts and the thick microtubules bundles in the developing flight muscles. Hair cells of the developing sensory organs also show strong expression, however, move out of the Z-stack over time. Video plays with 5 frames per second. Time is given in hh:mm.