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. 2016 Mar 14;5:e10860. doi: 10.7554/eLife.10860
Reagent Type Manufacturer Catalog # Comments
3T3-L1 cells Cell line ATCC CL-173 Original source unspecified
DMEM Cell culture Corning 15013 CV Replaces original from Invitrogen
FBS Cell culture CellGro 10437-028 Original cat # unspecified
KDM4C siRNA #1 Nucleic acid Synthesis left to the discretion of the replicating lab
KDM4C siRNA #2 Nucleic acid Synthesis left to the discretion of the replicating lab
KDM4C siRNA #3 Nucleic acid Synthesis left to the discretion of the replicating lab
Scrambled control siRNA Nucleic acid Dharmacon D-001810-01-20
Lipofectamine RNAiMAX Cell culture Invitrogen 13778-030 Original cat# unspecified
isobutylmethylxanthine Inhibitor Sigma I5879 Original cat# unspecified
dexamethasone Chemical Sigma D4902 Original cat# unspecified
insulin Growth factor Sigma I3536 Original cat# unspecified
Troglitazone Chemical Sigma T2573 Original cat# unspecified
RIPA buffer Cell culture Millipore 20188 Original source unspecified
Nitrocellulose membrane Western blot reagent Life Technologies LC2006 Original source unspecified
Ponceau stain Chemical Sigma P7170 Original unspecified
Mouse IgG2a monoclonal anti-β-actin Antibody Sigma A5316
Rabbit IgG monoclonal anti-H3 Antibody Cell Signaling Technology 4499
Rabbit polyclonal anti-H3K9me3 Antibody Abcam Ab8898
Rabbit polyclonal anti-KDM4C Antibody Abcam Ab85454
Oil-Red-O Chemical Sigma O1391 Original source unspecified
paraformaldehyde Chemical Tousimis 1008A Original source unspecified