3T3-L1 cells |
Cell line |
ATCC |
CL-173 |
Original source unspecified |
DMEM |
Cell culture |
Corning |
15013 CV |
Replaces original from Invitrogen |
FBS |
Cell culture |
CellGro |
10437-028 |
Original cat # unspecified |
KDM4C siRNA #1 |
Nucleic acid |
Synthesis left to the discretion of the replicating lab |
KDM4C siRNA #2 |
Nucleic acid |
Synthesis left to the discretion of the replicating lab |
KDM4C siRNA #3 |
Nucleic acid |
Synthesis left to the discretion of the replicating lab |
Scrambled control siRNA |
Nucleic acid |
Dharmacon |
D-001810-01-20 |
|
Lipofectamine RNAiMAX |
Cell culture |
Invitrogen |
13778-030 |
Original cat# unspecified |
isobutylmethylxanthine |
Inhibitor |
Sigma |
I5879 |
Original cat# unspecified |
dexamethasone |
Chemical |
Sigma |
D4902 |
Original cat# unspecified |
insulin |
Growth factor |
Sigma |
I3536 |
Original cat# unspecified |
Troglitazone |
Chemical |
Sigma |
T2573 |
Original cat# unspecified |
RIPA buffer |
Cell culture |
Millipore |
20188 |
Original source unspecified |
Nitrocellulose membrane |
Western blot reagent |
Life Technologies |
LC2006 |
Original source unspecified |
Ponceau stain |
Chemical |
Sigma |
P7170 |
Original unspecified |
Mouse IgG2a monoclonal anti-β-actin |
Antibody |
Sigma |
A5316 |
|
Rabbit IgG monoclonal anti-H3 |
Antibody |
Cell Signaling Technology |
4499 |
|
Rabbit polyclonal anti-H3K9me3 |
Antibody |
Abcam |
Ab8898 |
|
Rabbit polyclonal anti-KDM4C |
Antibody |
Abcam |
Ab85454 |
|
Oil-Red-O |
Chemical |
Sigma |
O1391 |
Original source unspecified |
paraformaldehyde |
Chemical |
Tousimis |
1008A |
Original source unspecified |