Figure 2.

Canonical Wnt-signaling demarcates dorsal forebrain germinal microdomains. (A) Overview of the β-Gal (β-galactosidase) intensity in canonical Wnt-report mouse line highlighting exclusive expression in the dSVZ and in the adjacent corpus callosum at postnatal day 4. For staining procedures (see Azim et al., 2014a). (B) Examples of pathway-specific target genes and signaling ligand expression gradients according to previous published studies (Azim et al., 2012, 2014a, 2015). FGF2 and Notch (Hes5) are homogeneously distributed in the P4 SVZ and gradients illustrate relative expression intensities based on previous qPCR validation.