Figure 3.

zein-hBMP2ad accumulates to higher levels than hBMP2nat in transgenic plants. Leaves (0.5 g, fresh weight) from independent transgenic tobacco plants expressing hBMP2nat (upper panel, lines 1–19), zein-hBMP2ad (lower panel, lines 1–11, 21–29) or untransformed plants (wt), were homogenized in Laemmli buffer at 95°C (ratio 7:1). Twenty-five microliters of leaf homogenate from each sample (corresponding to 12.5 μg of total leaf proteins) were subjected to SDS-PAGE, followed by protein blot analysis with anti-Flag antiserum. Protein blot exposure time: hBMP2nat, 15 min; zein-hBMP2, 1 min. Numbers on the left indicate the position and size (in kDa) of molecular mass markers.