Figure 4.

Both hBMP2nat and zein-hBMP2ad are soluble in tobacco transgenic plants. Thirty micrograms of total leaf proteins from wild type (wt, A, lanes 1–2) and hBMP2nat line #3 (A, lanes 3–4) plants or 3 μg of total leaf proteins from wild type (wt, B, lanes 1–2) and zein-hBMP2ad line #7 (B, lanes 3–4) plants, were extracted in the absence (−) or presence (+) of 2-ME and analyzed by SDS-PAGE and protein blot with anti-Flag antiserum. Asterisk: 18 kD hBMP2ad fragment. Numbers on the left indicate the positions of molecular mass markers, in kD.