Figure 5.

zein-hBMP2ad assembles both in dimers and in progressively larger multimers. Eight hundred micrograms of total leaf proteins from zein-hBMP2ad line #7 were extracted in the absence of 2-ME and fractionated by velocity sucrose (5–25% w/v) gradient centrifugation. Equal volume of fractions and the pellet at the bottom of the tube were analyzed by protein blot with anti-Flag antiserum. Asterisk indicates zein-hBMP2ad dimers. Arrow indicates the interface between stacking and separating gels. P: pellet at the bottom of the tube. Numbers on the top indicate the position along the gradient and the molecular mass (kD) of velocity centrifugation markers. Numbers on the left indicate the positions of molecular mass markers, in kD.