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. 2016 Mar 24;7:358. doi: 10.3389/fpls.2016.00358

Figure 6.

Figure 6

zein-hBMP2ad cofractionates with the ER. Hundred milligrams of fresh leaves from zein-hBMP2ad line #23 were homogenized in sucrose buffer, in the absence of detergent. The homogenates were fractionated by centrifugation on isopycnic sucrose gradient (16–65% w/w) in the presence of MgCl2 (left panels) or EDTA (right panels). Collected fractions were analyzed by SDS-PAGE followed by immunoblotting with anti-Flag antiserum (upper panels) or with a mix of anti-BiP and anti-Grp94 antisera (bottom panels). T, total extract; P, pellet at the bottom of the tube. Numbers at top indicate the density (g/ml) of sucrose. Numbers at left indicate the position of molecular mass markers, in kD.