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zein-hBMP2ad is N-glycosylated. Eight micrograms of total leaf proteins from zein-hBMP2ad line #7 or from wt plants, extracted in the presence of 2-ME, were incubated with Endo H (+) or without enzyme (−) as control and analyzed by SDS-PAGE and protein blot with anti-Flag antiserum. Bottom panel: longer exposure of the 14–18 kD portion of the blot. Numbers at left indicate the position of molecular mass markers, in kD.
