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. 2016 Mar 24;7:358. doi: 10.3389/fpls.2016.00358

Figure 8.

Figure 8

Quantification of zein-hBMP2ad expressed in tobacco plants. Sequential dilutions of total leaf proteins (from 0.5 to 4 μg) from zein-hBMP2ad line #23 extracted in the presence (+2-ME) or in the absence (−2-ME) of 2-mercaptoethanol were analyzed by protein blot in comparison with known amounts of the commercial standard protein Flag–BAP (lanes on the left). Empty circle: zein-hBMP2ad monomers; asterisk: zein-hBMP2ad dimers. Numbers at left indicate the position of molecular mass markers, in kD.