Table 1.
Morphological changes in 3O-C12-HSL-stimulated macrophages identified by 2D live imaging.
| Time (min) | Control | 10 μM 3O-C12-HSL | 50 μM 3O-C12-HSL | HTS13268 | HTS13268 and 50 μM 3O-C12-HSL |
|---|---|---|---|---|---|
| POLARIZED MACROPHAGES (% OF TOTAL CELLS) | |||||
| 1 | 44±7 | 53±7 | 54±5 | 33±5 | 34±10¤ |
| 10 | 41±7 | 35±3*# | 51±5 | 25±5 | 31±11¤ |
| 15 | 44±6 | 47±4 | 46±4 | 25±5* | 30±10* |
| 30 | 40±7 | 37±2*# | 41±6§ | 30±6 | 29±10 |
| 90 | 43±7 | 35±3*# | 39±4#§ | 33±6 | 20±5*¤ |
| 180 | 41±6 | 52±12 | 38±5# | 28±8 | 21±5*¤ |
| MACROPHAGES WITH ONE OR MORE DISTINCT LAMELLIPODIA (% OF TOTAL CELLS) | |||||
| 1 | 43±4 | 50±5 | 49±7 | 36±3 | 45±8 |
| 10 | 38±5 | 22±11# | 41±4§ | 29±8 | 38±12 |
| 15 | 35±4 | 22±12# | 46±4*§ | 28±8 | 30±13 |
| 30 | 38±5 | 28±2## | 47±5§ | 31±9 | 28±7¤ |
| 90 | 44±6 | 37±9 | 41±5 | 34±7 | 28±6 |
| 180 | 45±6 | 42±15 | 43±7 | 46±13 | 30±8 |
Significant differences were analyzed by one-tailed unpaired Student's t-test and are indicated with:
*
compared to control at the same time point;
#
within the group (eg. 50 μM 3O-C12-HSL) compared to the 1 min-value in the group;
¤
50 μM 3O-C12-HSL vs. 50 μM 3O-C12-HSL+HTS13286;
§
10 μM 3O-C12-HSL vs. 50 μM 3O-C12-HSL.