Figure 1.

Functional characterization of microparticles (MPs) by Laser Scanner Confocal Microscopy (LSCM). Schematic representation of the LSCM method allowing individual MPs immobilization, characterization (including labeling and functional studies). Step 1-annexin-V coating of the micro-wells. Step 2- PS+-MPs (CFSE+) immobilization on the coated surface in the presence of Ca²⁺ thanks to the high affinity binding of PS present to annexin-V. Step 3- Immobilized MPs characterization: 3A. Morphological MPs characterization, including number and size distribution. 3B. Immunofluorescence analyses, using specific primary (against proteins present on the MP surface) and secondary-labeled antibodies. 3C. Proteolytic activity studies (nano-zymography), after addition of specific fluorogenic substrates. PS=Phosphatidylserine. LSCM=laser scanning Confocal Microscopy.