Figure 3.

Laser Scanner Confocal Microscopy of immobilized MPs from THP-1 cells allows measurement of MP size and concentration. (A) Dose-dependent detection of THP-1-derived MPs (CFSE+, green) in the presence or in the absence of Ca²⁺. (n=3) (B) CFSE+ MPs quantification according to their concentration and the presence (black) or absence (grey) of Ca²⁺. (n=3) (C) CFSE+ MPs distribution depending on their size, including the apparent median diameter (0.43 µm). (n=4) (D) Loss of CFSE staining by THP-1 MPs after destruction by a detergent (Triton X-100, 1%) as compared to control-untreated MPs. (n=3) (Bars, 10µm). * p<0.05 vs Control. Statistical analyses were performed using Mann-Whitney's U-test.