Figure 4.

Cerenkov luminescence imaging with ²²⁵Ac-DOTA-c(RGDyK). Images were acquired 24 h after intravenous injection to allow for equilibrium to occur between ²²⁵Ac and its daughter products, which generate Cerenkov luminescence. (A) Efficient tumor localization was observed in U87mg tumor bearing animals at 24 h p.i. (B) Localization was reduced upon administration of blockade demonstrating in vivo specificity. (C, D) In vivo (C) and ex vivo (D) quantification based upon ROI analysis demonstrates that Cerenkov radiation in the tumor is significantly reduced upon the administration of blockade. (★) denotes tumor.