Figure 2.

Proof of function of scuPA constructs. A. Urokinase activity assay of scuPA on 96-well plates using urokinase substrate S2444L. Increase of absorption at 405nm was measured over a 40 min period. ScuPA and standards using commercial uPA at different concentrations resulted in linear enzymatic activity. B. Plasminogen conversion assay of scuPA on 96-well plates using S2251 amidolytic assay. The conversion of plasminogen to plasmin was monitored with the S2251 amidolytic assay. Generation of plasmin for scuPA at 10nmol/L was compared against standards of commercial uPA at different concentration. Increase of absorption at 405nm was measured over a 40 min period. C. Activity of scuPA was calculated from the initial velocities obtained from lines fitted to data of time versus plasmin generated for the standards at a range of 0 to 15U/ml commercial uPA.