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. 2016 Mar 20;6(5):726–738. doi: 10.7150/thno.14514

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Flow cytometry demonstrating the functionality of scFv_anti-LIBS and efficiency of in vivo biotinylation. A. Functionality of scFv_anti-LIBS was proven with an AlexaFluor 488-coupled anti-Penta-His antibody in flow cytometry. Bar graphs depict the median fluorescence intensity values of five independent experiments. Representative fluorescence histograms are shown underneath the bar graphs. B. Functionality of scFv_anti-LIBS as well as the efficiency of in vivo biotinylation was evaluated using R-phycoerythrin streptavidin in flow cytometry (mean ± SD, ***p<0.001). These assays were analyzed using a paired t-test. C. Representative microscopy images of targeted-theranostic microbubbles (TT-MBs) attached to activated platelets on microthrombi in a flow chamber experiment.