Fig. 2.

The effect of riboflavin on TNF-α release (left panel) in the second hour of inflammation and IL-6 (right panel) in the 6th hour of inflammatory process induced by i.p. administration of zymosan (40 mg/kg) at BALB/c (a) and C57BL/6J mice (b) and by RAW 264.7 macrophages (c) stimulated with zymosan (250 µg/ml). The inflammation was modulated by riboflavin supplementation (50 mg/kg for mice or 25 µg/ml for RAW 264.7 macrophages) 30 min prior to zymosan, simultaneously with zymosan or 2, 4 h after zymosan. The results are presented as mean values with a standard deviation. N = 4; p < 0.05. Statistically significant differences determined relative to control values are marked with the following symbols over the bars: *p < 0.05; **p < 0.01