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. 2016 Mar 23;18:86. doi: 10.1007/s11051-016-3389-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Three tiers of toxicological analysis for SAEC illustrated with respect to WST-1 viability assay. In tier 1, the data are grouped. Particle material, dose, and morphology are disregarded in the analysis. In tier 2, the data are differentiated regarding particle material, but dose and morphology are still disregarded. In tier 3, dose and morphology are also considered and a possible dose–response relation is investigated. For Cu and Ag, series 1 and 2 are pooled for all statistical analysis