Table 3.
SQR activity in PF RNAi SDH1 noninduced (N) and tetracycline induced (+tet) cells in the presence of absence of malonate (mal).
| Cells | SDH specific activity (Ua/mg) ± SEMb | % of 29–13 activity | % of RNAi N activity |
|---|---|---|---|
| PF 29-13 | 0.658 ± 0.021 | 100 | |
| PF 29-13 + mal | 0.156 ± 0.004 | 24 | |
| PF RNAi Sdh1 N | 0.497 ± 0.005 | 100 | |
| PF RNAi Sdh1 N/+mal | 0.107 ± 0.008 | 22 | |
| PF RNAi Sdh1 1d +tet | 0.19 ± 0.0046 | 38 | |
| PF RNAi Sdh1 3d +tet | 0.08 ± 0.005 | 16 |
The unit (U) of SQR is defined as an amount of enzyme required for conversion of 1 nmol of 2,6-dichlorophenolindophenol/min/mg of mitochondrial proteins.
Each measurement was performed in triplicate.