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. 2016 Jan 5;110(1):103–112. doi: 10.1016/j.bpj.2015.11.027

Figure 1.

Figure 1

Range of observable sedimentation coefficients as a function of time for different rotor speed schedules. (Top) Rotor speed models using a constant field increase model (blue), a power-law model (red), an ad hoc model consisting of two lower speed steps at 5000 and 10,000 rpm followed by a constant field increase (green dashed), and a model with slowly linearly increasing rotor speed (orange dashed). Rotor speed changes were initiated in 45-min intervals, with rotor acceleration of 280 rpm/s, with ωlow = 3000 rpm and ωmax = 60,000 rpm. For comparison, constant speed data are shown for a conventional constant speed experiment at 50,000 rpm (black solid) and 3000 rpm (cyan solid). (Bottom) Observable range of s values for nondiffusing particles located between 6.1 and 7.1 cm in a 12 mm column, indicated with patches colored corresponding to the rotor speed schedules on the top. To see this figure in color, go online.