Figure 7.

Sufficiency of eNOEs in defining χ¹ angles. Angles obtained from ensembles calculated from eNOEs exclusively are shown on the left, and ensembles calculated from eNOEs, RDCs, and J couplings on the right. The four states of 20 ensembles are plotted from the center to the outer circle in the circle diagrams. The red bars indicate angles extracted from the high-resolution x-ray structure 1IGD (45), and the green and yellow bars different states and additional states from an anisotropic reevaluation. Angles for which an additional state is obtained or a state disappears are shaded in light blue. The single-letter amino acid codes and residue numbers are colored according to the solvent-accessible surface area of the residue (red for 0% and blue for 50% or more). The figure was generated in MolMol (59). To see this figure in color, go online.