Table 1.
Conditions for measuring β-glucuronidase in culture medium
| Medium (mL) | Water (mL) | Acetate buffer (mL) | pH | Substrate final | Molarity (mL) | Incubation time at 37 °C (h) | Alkalinizing reagent (mL) | Final pH | Coloremeter wavelength (nm) |
| 0.2 | 0.4 | 0.2 | 4.5 | 0.2 | 0.006 | 18 | 21 + 3 mL H2O | 10.2 | 540 |
1
Glycine-Duponal reagent: 15.01 g of glycine dissolved in 900 mL of H2O and brought to pH 11.7 by addition of 50% NaOH solution. Duponal (sodium lauryl sulfate) was added to produce a final concentration of 0.2% and water was added to achieve a final volume of 1 L.