Figure 2. Pum1-P3E interaction is required for both auto-repression and support of filamentation.

Equal number of α and a mating partners of wild type, pum1Δ, mycPUM1, mycPUM1-EM and mycPUM1-RBD were cocultured and spotted on to the V8 mating media and incubated in the dark at room temperature. Photographs were taken at the indicated times.