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. 2016 Mar 24;6:23472. doi: 10.1038/srep23472

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(A) Screening of in-house small molecule library for inducing miR-182. Cells were treated with 10 μM of each small molecule for 24 hours. Relative miR-182 expression was measured by real-time PCR. (B) Cells were exposed to hypoxia with varying concentrations of kenpaullone treatment. miR-182 expression was measured by real-time PCR. D: DMSO. *p < 0.05 (C) The expression of BNIP3 and apoptosis-related genes under hypoxia with or without kenpaullone (Ken, 10μM) treatment was evaluated by western blot analysis. (D) Representative images of mitochondrial fission induced by hypoxia with or without kenpaullone treatement. (E) Cellular apoptosis was evaluated by flow cytometry. Ken: kenpaullone.