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. 2016 Mar 24;6:23665. doi: 10.1038/srep23665

Figure 3. Metformin-induced Igfbp-2 gene expression is mediated by AMPK.

Figure 3

(A) Igfbp-1 and Igfbp-2 mRNA expressions in mouse primary hepatocytes treated with metformin and compound C (Com C) for 12 h. (B) Effect of metformin and compound C (Com C) on the expression of IGFBP-2 and AMPK in primary hepatocytes. The bar graph on the right shows the quantification of IGF-1 phosphorylation over total IGF-1. (C) Effect of metformin on IGFBP-2 protein level when Ampk or Sirt1 was knocked down. AML12 cells were infected with Ad-si Ampkα2 and Ad-si Sirt1 for 36 h and then treated with metformin for 24 h. IGFBP-2, Sirt1 and AMPK phosphorylation in AML12 cell line for the indicated conditions were detected by immunoblotting. *p < 0.05, **p < 0.01 vs. untreated control and/or metformin-treated cells. (D) Relative hepatic Igfbp-2, Sirt1, and Ppara mRNA level in the primary hepatocytes from WT and Ppara null mice transfected with Ad-GFP or Ad-Sirt1. Mouse primary hepatocytes from WT and Ppara null mice were infected with Ad-GFP or Ad-Sirt1 for 24 h and then treated with metformin for 12 h. ***p < 0.001 vs. WT mice.