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. 2016 Mar 24;6:23727. doi: 10.1038/srep23727

Figure 4. The effect of E2 on autophagosome number.

Figure 4

Western blotting analysis and relative densitometric analyses of LC3 and p62 cellular levels in MCF-7 cells treated with E2 at different time points (a,a’) or treated for 120 min with E2 in the presence or in the absence of bafilomycin A1 (Baf) (100 nM–2 hrs) (c,c’). For LC3 quantitation, the formula LC3-II/(LC3-I + LC3-II) has been applied. The loading control was done by evaluating vinculin expression in the same filter. *indicates significant differences with respect to the control (−) sample; °indicates significant differences with respect to the corresponding E2 sample. (b) LC3 (red signal) and monodansylcadaverine (MDC) (green signal) immunofluorescence staining in MCF-7 cells treated with E2 (10 nM) for 120 min. The scale bar represents 10 microns.