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. 2015 Mar 5;1(1):e4. doi: 10.1097/TXD.0000000000000511

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Copyright © 2015 The Authors. Transplantation Direct. Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Cell numbers after reactivation. Four million thawed Treg were plated for 48 hours in each of 5 conditions: media plus (1) no additives, (2) IL-2, (3) anti-CD3, (4) IL-2 + anti-CD3, and (5) IL-2 + anti-CD3 + L cells (artificial APCs transfected with human CD58, CD32, and CD80). Average cell numbers and standard deviation for each condition after 48 hours are shown. This experiment was performed 5 times using 3 unique Treg lines. NS: non-significant. *: P < 0.05 by ANOVA. ANOVA, analysis of variance.