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. 2016 Mar 24;6:23590. doi: 10.1038/srep23590

Figure 6. Nck is differentially distributed in the absence of WIP or WIRE.

Figure 6

shControl, shWIP and shWIRE MDA-MB-231 cells were allowed to invade rhodamine-gelatin (red, 5 h). (a) Cells were fixed and stained for F-actin (green) and Nck (cyan). Bars: 10 μm. (b) Normalized contour map from insets in (a), dark tones indicate high protein expression and light tones, low expression. (c) Profiles of fluorescence intensity of F-actin (green), Nck (blue) and gelatin (red). Profiles were traced in areas containing invadopodia (when present) and represent average measurements of a 10-pixel range. PM =  plasma membrane, PN =  perinuclear, IN =  invadopodium.