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. 2016 Mar 24;6:23555. doi: 10.1038/srep23555

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Photographs in the top and middle rows for each chromatin configuration are the same oocyte observed with phase contrast and fluorescence, respectively, after Hoechst 33342 staining. The nucleolus is indicated with arrows in the phase contrast images. Original magnification ×400. For convenience, GV-0 was designated as NSN configuration, and GV1, GV2 and GV3 were classed as SN configuration in the present study. Photographs in the bottom row are laser confocal (merged) images showing global RNA transcription of porcine oocytes with different GV chromatin configurations. DNA and RNA were pseudo colored blue and red, respectively. Original magnification ×630. Each treatment was repeated 3 times with each replicate containing about 30 oocytes.