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. 2016 Mar 24;6:23555. doi: 10.1038/srep23555

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Copyright © 2016, Macmillan Publishers Limited

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(A,B) show p-MAPK and Npr2 mRNA, respectively, in CCs from COCs that had been cultured for 16 h with (+db) or without db-cAMP. (C–E) show p-MAPK, Npr2 mRNA and cAMP, respectively, in CCs from follicles of different diameters. Each treatment was repeated 3 times with each replicate containing CCs from 200 COCs for p-MAPK and Npr2 mRNA assays and about 2 × 10⁵ CCs for cAMP measurement. (a,b) Values without a common letter above their bars differ significantly (P < 0.05).