Table 1.

List of primers used to construct GFP-expressing plasmid, pBS-EGRADE

Primer	Sequence
BoefIG2-F-ClaI-IF	AGGTCGACGGTATCGATCACTCATTTAGATTGCGA
BoefIG2-R-ClaI-IF	ATATCAAGCTTATCGATCTTGTTTAAGGTTTAACGA
eGFP-F-HindIII-IF	CAAGATCGATAAGCTTATGGTGAGCAAGGGCGAGGA
eGFP-R-HindIII-IF	TCCCGATATCAAGCTTTTACTTGTACAGCTCGTCCA
BoRAPIG-F-EcoRV-IF	ATCGATAAGCTTGATATCGGGAGCAGAGCTCTGGCCA
BoRAPIG-R-EcoRV-IF	CTGCAGGAATTCGATATCCGTAAAGCGAGACGGGGA
BoActin5UTR-F-SmaI-IF	GAATTCCTGCAGCCCGGGCACATAAAGGTACCTATGCT
BoActin5UTR-R-SmaI-IF	ACTAGTGGATCCCCCGGGCTTGGCGAAATTTTACTCCT
hDHFR-F-BamHI-IF	CAAGCCCGGGGGATCCATGGTTGGTTCGCTAAACT
hDHFR-R-BamHI-IF	ACGTACTAGTGGATCCTTAATCATTCTTCTCATATAC
Boef3UTR-F-SpeI-IF	CGGGGGATCCACTAGTACGTTGCGCCTTTAGGTGCA
Boef3UTR-R-SpeI-IF	CCGCTCTAGAACTAGTGGTGTGAGCATTGCTTGTC
BoADE-F-EcoRI-IF	TACGGATATCGAATTCCTGCAGCCCGGGCACATAAAGG
BoADE-R-EcoRI-IF	CGGGCTGCAGGAATTCACTAGTGGTGTGAGCATTGC

Restriction enzyme sites are underlined