Table 3.
MS-MLPA analysis on the FMR1 locus before and after 7-day treatment with 5-azadC (T1) of three different normal control female cell lines (WTA, B and C)
Probes | WTA UT ratio | WTA T1 ratio | WT B UT ratio | WT B T1 ratio | WTC UT ratio | WTC T1 ratio |
---|---|---|---|---|---|---|
Prom | 0.48 | 0.38 | 0.52 | 0.3≪ | 0.52 | 0.37≪ |
Prom | 0.47 | 0.25 | 0.42 | 0.32 | 0.53 | 0.25 |
Exon 1 | 0.33 | 0.26 | 0.27 | 0.3 | 0.39 | 0.28 |
Intron 1 | 0.56 | 0.36 | 0.52 | 0.19≪ | 0.68 | 0.42 |
Intron 1 | 0.25 | 0.19 | 0.3 | 0.4 | 0.38 | 0.23 |
Intron 1 | 0.36 | 0.24 | 0.35 | 0.25 | 0.35 | 0.25 |
Intron 1 | 0.35 | 0.24 | 0.3 | 0.25 | 0.39 | 0.26 |
Exon 7 | 0.98 | 0.46 ≪* | 0.98 | 0.6 ≪* | 0.93 | 0.58 ≪* |
Exon 17 | 0.97 | 0.9 | 0.94 | 0.93 | 0.97 | 0.88 |
Exon 1 AFF2 | 0.52 | 0.45 | 0.54 | 0.43≪ | 0.61 | 0.42≪ |
Exon 1 AFF2 | 0.49 | 0.37 | 0.42 | 0.33 | 0.42 | 0.33 |
Exon 1 AFF2 | 0.23 | 0.23 | 0.3 | 0.19≪ | 0.34 | 0.24 |
Exon 1 AFF2 | 0.46 | 0.3 | 0.49 | 0.36 | 0.53 | 0.38 |
Exon 1 AFF2 | 0.27 | 0.17 | 0.22 | 0.14 | 0.38 | 0.23 |
Xp22 | 0.41 | 0.34 | 0.38 | 0 | 0 | 0 |
Only methylation-sensitive probes are listed
Note that the asterisks indicate that the magnitude of the probe ratio exceeds the set of arbitrary border values. The italic values represent levels of methylation lower than 30 %, bold italic values represent those between 30 and 70 %, while underlined values represent those higher than 70 %