Fig. 1.

Retinal I/R injury significantly increases C1q expression in the retina. C57BL/6 J mice were subjected to unilateral I/R injury and sacrificed at the indicated time points post I/R. Contralateral control eyes (a) displayed basal deposits of C1q in the GCL; however, ischemic eyes (b–f) had significantly greater expression of C1q as early as day 3 (b) and sustained to the end of the time course on day 28 (f). Positive labeling was only observed in the GCL and IPL. (g) Fluorescence intensity quantification revealed statistically significant upregulation of C1q (p < 0.01) at all time points in ischemic versus uninjured retinas. Peak intensity in ischemic eyes was observed on day 21 post injury (p < 0.05). Mean ± SEM, n = 7 per group. **p < 0.01 determined by student’s paired t-test, #p < 0.05 tested using One-way ANOVA followed by Holm-Sidak post test. GCL = Ganglion Cell Layer, INL = Inner Nuclear Layer, ONL = Outer Nuclear Layer. Scale bars = 50 μm