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. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Am J Obstet Gynecol. 2015 May 12;213(3):387.e1–387.10. doi: 10.1016/j.ajog.2015.05.007

FIGURE 3. Time-course of placental and fetal brain IL-6 RNA levels after dam exposure to increasing doses of LPS.

FIGURE 3

Representative gel of amplicons that were obtained from A, placenta tissue or B, fetal brain 2 hours after maternal LPS injections. In each gel, products that were obtained from amplification reactions with IL-6 primers are shown on the left; products that were obtained with β-actin primers are shown on the right. Lanes are labeled c(controls) and i, ii, iii (160, 320 and 640 μg/kg LPS), respectively. Graphs depict IL-6 RNA expression levels in placenta and fetal brain relative to expression at 0 hours with LPS doses of 640 μg/kg (squared bars), 320 μg/kg (vertical line bars), 160 μg/kg (horizontal line bars) and 0 μg/kg (open bars). Data are expressed as mean ± SD. One-way analysis of variance with Least Significant Difference Post-Hoc test was performed; a probability value of ≤ .05 was set as a significance threshold. A, The single asterisk indicates a probability value of ≤ .05; the double asterisk indicates a probability value of ≤ .01 vs saline solution; the triple asterisk indicates no significance compared with 640 μg at 2 hours or 160 μg at 6 hours. B, The single asterisk indicates a probability value of ≤ .05 vs saline solution. C, Distribution of fold IL-6 expression normalized to β-actin in individual pup brains from the 640 μg and control groups is shown.