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. 2015 Nov;96(Pt 11):3444–3455. doi: 10.1099/jgv.0.000281

Fig. 2.

Fig. 2.

Infectivity titres of serially diluted E2 CWD brain isolate. Groups of 10 mice were inoculated intracranially with the E2 isolate of CWD prions diluted into normal brain homogenate at the indicated serial dilutions. End-point prion titres were determined as described previously (Reed & Muench, 1938). Error bars are smaller than the indicated points for mean infectivity on the graph. Dashed line indicates extrapolated values that lie outside of the dynamic range of the bioassay. Dotted lines converging from y = 100 and x = 10− 11.5 represent the extrapolated dilution of E2 brain homogenate (10− 11.5) containing 1 LD50 U (100) (g tissue)− 1. Dotted lines converging from x = 10− 12 and y = 10− 0.5 represent the extrapolated infectivity titre [0.32 LD50 U (g tissue)− 1] of a 10− 12 dilution of E2 brain homogenate that is the PMCA seeding dose for seven rounds of PMCA.