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. 2015 Nov;96(Pt 11):3444–3455. doi: 10.1099/jgv.0.000281

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Fig. 6. — PrP^CWD detection in elk fetal tissues following seven rounds of sPMCA. Representative Western blots for detection of PrP^CWD in fetal tissues. sPMCA controls showed the lack of amplification for a CWD-negative sample and the successful amplification of a known CWD-positive white-tailed deer. Western blot controls (no sPMCA) showed complete proteinase K (PK) digestion of PrP in a negative white-tailed deer brain homogenate and PrP^CWD signal post-proteinase K digestion in a CWD-positive white-tailed deer brain homogenate (10 % homogenate, undiluted, no sPMCA). Tissue codes: C, control (+, positive; –, negative); BR, brain; CL, colon; IT, intestine; LNG, lung; LVR, liver; PLN, popliteal lymph node; RLN, retropharyngeal lymph node; RT, rectum; SP, spleen; TY, thymus; UM, umbilicus; VG, vagus nerve. Asterisks denote sPMCA+ CWD amplification.

Fig. 6. — PrP^CWD detection in elk fetal tissues following seven rounds of sPMCA. Representative Western blots for detection of PrP^CWD in fetal tissues. sPMCA controls showed the lack of amplification for a CWD-negative sample and the successful amplification of a known CWD-positive white-tailed deer. Western blot controls (no sPMCA) showed complete proteinase K (PK) digestion of PrP in a negative white-tailed deer brain homogenate and PrP^CWD signal post-proteinase K digestion in a CWD-positive white-tailed deer brain homogenate (10 % homogenate, undiluted, no sPMCA). Tissue codes: C, control (+, positive; –, negative); BR, brain; CL, colon; IT, intestine; LNG, lung; LVR, liver; PLN, popliteal lymph node; RLN, retropharyngeal lymph node; RT, rectum; SP, spleen; TY, thymus; UM, umbilicus; VG, vagus nerve. Asterisks denote sPMCA+ CWD amplification.