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editorial
. 2015 Nov;161(Pt 11):2174–2183. doi: 10.1099/mic.0.000179

Fig. 1.

Fig. 1.

Y. pestis expressing Ail binds vitronectin. (a) Co-sedimentation of vitronectin from NHS or HIS with Y. pestis KIM8-E (Yp Ail+) and Y. pestis KIM8-E Δail (Yp Δail). Bacteria were incubated with 50 % NHS or HIS at 4 °C for 2 h. Washed cells and co-sedimenting proteins were subjected to SDS-PAGE and immunoblotting with VIT-2 anti-vitronectin mAb (α-Vn mAb), anti-FLAG M2 mAb (α-FLAG) or anti-Ail (α-Ail) antisera. The 65 and 75 kDa vitronectin bands are marked by arrows. Complementation of Y. pestis KIM8-E Δail with plasmid pFLAG-Ail restores expression of Ail and binding of vitronectin. In contrast, expression of the pFLAG-ATS vector or pFLAG-OmpX does not restore vitronectin binding. (b) Co-sedimentation of purified monomeric vitronectin with Y. pestis KIM8-E (Yp Ail+) and Y. pestis KIM8-E Δail (Yp Δail). Bacteria were incubated with pure vitronectin (50 μg ml–1) at 37 °C for 1 h and washed cells subjected to immunoblot analysis with anti-vitronectin mAb (α-Vn mAb), FLAG M2 mAb (α-FLAG) or anti-Ail (α-Ail) antisera. MW, molecular mass standard.