Fig. 4.

(R)-3-HB induction of PA2004-lacZ required the PA2005 gene. bdhA : : Tn, PA2004 : : Tn, PA2005 : : Tn and WT P. aeruginosa PAO1 harbouring the PA2004-lacZ reporter were grown in (a) LB or M63 minimal medium that was supplemented with either (b) 30 mM succinate or (c) 30 mM acetate. At an OD₆₀₀ of 0.3, (R,S)-3-HB was added to a final concentration of 30 mM, and LacZ activity was determined 1, 2, 3 and 4 h post-induction. LacZ activity increased for bdhA : : Tn, PA2004 : : Tn and WT cells when challenged with (R,S)-3-HB. In contrast, (R,S)-3-HB did not induce LacZ activity in the PA2005 : : Tn mutant under any growth condition. Data points represent mean values ± sd (n = 4). ANOVA was performed using a Dunnett's post-hoc test (α-value of 0.05) to identify significant changes (P < 0.0001).