Table 3. Gene expression stability of the nine candidate reference genes under different conditions calculated by NormFinder.
Total | Developmental stages | Biotic stress | Drought stress | Salinity stress | Cold stress | Heat stress | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene | NF | Gene | NF | Gene | NF | Gene | NF | Gene | NF | Gene | NF | Gene | NF |
TIP41 | 0.382 | TIP41 | 0.158 | CLA | 0.058 | CYP | 0.117 | TIP41 | 0.054 | SAND | 0.052 | CYP | 0.073 |
EF1 | 0.413 | ACT | 0.268 | SAND | 0.073 | 18S rRNA | 0.139 | EF1 | 0.073 | CYP | 0.090 | TIP41 | 0.164 |
BHLH | 0.436 | EF1 | 0.269 | ACT | 0.096 | GAPDH | 0.145 | CLA | 0.074 | GAPDH | 0.115 | EF1 | 0.206 |
SAND | 0.469 | SAND | 0.331 | EF1 | 0.154 | TIP41 | 0.177 | GAPDH | 0.083 | TIP41 | 0.173 | CLA | 0.240 |
GAPDH | 0.483 | GAPDH | 0.414 | BHLH | 0.215 | BHLH | 0.199 | BHLH | 0.092 | EF1 | 0.329 | GAPDH | 0.282 |
CLA | 0.657 | 18S rRNA | 0.470 | GAPDH | 0.218 | SAND | 0.258 | SAND | 0.270 | CLA | 0.348 | ACT | 0.315 |
ACT | 0.768 | CLA | 0.620 | TIP41 | 0.353 | EF1 | 0.265 | CYP | 0.418 | ACT | 0.349 | SAND | 0.362 |
18S rRNA | 1.068 | BHLH | 0.915 | CYP | 0.419 | ACT | 0.311 | 18S rRNA | 0.437 | BHLH | 0.383 | BHLH | 0.455 |
CYP | 1.085 | CYP | 1.789 | 18S rRNA | 0.543 | CLA | 0.333 | ACT | 0.478 | 18S rRNA | 0.385 | 18S rRNA | 0.717 |
Note:
A lower normalization factor (NF) value indicates higher stability of the gene.