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. 2016 Mar 21;4:e1835. doi: 10.7717/peerj.1835

Figure 5. pERK signalling responses in stably and transiently receptor-expressing HEK cells.

Figure 5

Time courses (A, C, E, G) are representative data from single experiments, normalised for pERK level induced by treatment with 100 nM PMA for 4 min 30 s (100%) and 10 µM U0126 for 30 min (0%). Area-under-the-curve analysis (B, D, F, H) is combined data from three biological replicates (B and D) or two biological replicates (F and H). (A) and (B) show pERK stimulation of HEK cells stably expressing 3HA-hCB1 receptors, (C) and (D) show pERK stimulation of HEK Flp-in cells stably expressing HA-hGPR18, (E) and (F) show pERK stimulation of HEK Flp-in WT cells transiently expressing HA-hGPR18 pCAG (receptor-expressing subpopulation only), and (G) and (H) show pERK stimulation of the same HEK Flp-in WT cells, but for the subset of cells not expressing receptor.

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