Figure 6. pplss-3HA-hGPR18 HEK Flp-in cell expression and signalling.

(A) Is combined data from three biological replicates, comparing surface and total receptor expression in the pplss-3HA-hGPR18 Flp-in HEK cell line relative to the 3HA-hCB1 HEK stable cell line. Summary data (B, C) is area-under-the-curve analysis performed for combined data from three biological replicates. (B) shows pERK stimulation in pplss-3HA-hGPR18 Flp-in cells positive for GPR18 expression (based on anti-HA co-labelling), while (C) shows pERK stimulation in the same experiment but from cells that do not stain for receptor.