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Changes to receptor surface expression (in comparison with vehicle) following stimulation with high concentrations of putative ligands, at time points of 15 min (A, B), 1 h (C, D) and 18 h (E, F) for HEK Flp-in cells stably expressing pplss-3HA-hGPR18 (A, C, E, G) and HEK cells stably expressing 3HA-hCB1 (B, D, F, H). (G and H) show changes in total expression (in comparison with vehicle) at 18 h (n = 3).
