Skip to main content
. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Biochemistry. 2016 Mar 3;55(10):1485–1493. doi: 10.1021/acs.biochem.5b01364

Figure 2.

Figure 2

Relative equilibrium tryptophan fluorescence (excitation at 280 nm) of the DHFR apoenzyme (red), DHFR·folate (yellow), DHFR·NADP+ (blue), and DHFR·NADP+·folate (green). These spectra were recorded at 20 °C with 3 μM enzyme.