Table 2:

The point-of-care (POC) device validation studies used in this study, grouped by class

Citation	Species	Temperature (°C)	POC device used	Standard method	Analyte measured	Comparison with POC reading	Acceptable comparison
Awruch et al. (2011)	Shark (Galeorhinus galeus)	Not available	Lactate Pro	Enzymatic kit/spectrophotometer	Lactate	Similar	Yes
Gallagher et al. (2010)	Sharks (Carcharhinus plumbeus, Mustelus canis)	25	i-STAT (CG4+)	Blood gas analyser (thermostatted)	pH	Similar	Yes
				Blood gas analyser (thermostatted)	pO2	Similar	Yes
				Blood gas analyser (thermostatted)	pCO2	Similar	Yes
				Laboratory lactate and glucose analyser	Lactate	Similar	Yes
Brown et al. (2008)	Bony fish (Gadus morhua)	Not available	Lactate Pro	Enzymatic kit/spectrophotometer	Lactate	Similar	Yes
Clark et al. (2008)	Bony fish (Oncorhynchus nerka, Oncorhynchus tshawytscha, Thunnus orientalis, Scomber japonicus)	Not available	HemoCue	Drabkin method	Haemoglobin	Higher	Somewhat
Cooke et al. (2008)	Bony fish (Albula vulpes)	21–25	i-STAT (E3+)	Laboratory chemistry analyser	Na+	Higher	Yes
					K+	Lower	Yes
					Cl−	Lower	Somewhat
				Centrifuge	Haematocrit	Variable	Yes
			Accu-Chek Advantage	Laboratory chemistry analyser	Glucose	Similar	Yes
DiMaggio et al. (2010)	Bony fish (Fundulus seminolis)	Not available	i-STAT (E3+)	Centrifuge	Haematocrit	Lower	No
				Flame photometer	Na+	Lower	No
				Flame photometer	K+	Lower	No
				Chloridometer	Cl−	Higher	No
Evans et al. (2003)	Bony fish (Oreochromis niloticus)	25–28	One Touch Ultra	Laboratory colorimetric method	Glucose	Lower	Yes
Harrenstien et al. (2005)	Bony fish (Sebastes melanops, Sebastes mystinus)	11.5	i-STAT (EC8+)	Laboratory chemistry analyser	Na+	Lower	Somewhat
				Laboratory chemistry analyser	K+	Similar	No
				Laboratory chemistry analyser	Cl−	Variable	No
				Laboratory chemistry analyser	BUN	Lower	Somewhat
				Laboratory chemistry analyser	Glucose	Similar	No
				Laboratory chemistry analyser	Haemoglobin	Lower	Somewhat
				Blood gas analyser	pH	Lower	Somewhat
				Blood gas analyser	pCO2	Higher	Somewhat
				Blood gas analyser	TCO2	Lower	No
				Blood gas analyser	HCO3−	Lower	No
				Blood gas analyser	Base excess	Lower	No
Iwama et al. (1995)	Bony fish (Salmo salar)	Not available	ExacTech	Laboratory assay kit	Glucose	Similar	Yes
			BMS Hemoglobinometer	Laboratory assay kit	Haemoglobin	Similar	Yes
			Ames minilab	Laboratory assay kit	Glucose, haemoglobin	Similar	Yes
Serra-Llinares et al. (2012)	Bony fish (Gadus morhua)	4	Lactate Pro	Reference values	Lactate	Similar	Yes
Wells and Pankhurst (1999)	Bony fish (Oncorhynchus mykiss)	14–40	Accuchek Advantage	Hexokinase method	Glucose	Lower	Somewhat
			Accusport	Enzymatic kit/spectrophotometer	Lactate	Lower	Somewhat
McCain et al. (2010)	Reptiles (Pogona vitticeps, Tiliqua gigas, Geochelone platynota, Geochelone elegans, Boa constrictor, Pituophis melanoleucus)	Not available	i-STAT (6+)	Laboratory chemistry analyser	Na+	Similar	Somewhat
					K+	Similar	Somewhat
					Cl−	Similar	Somewhat
					Glucose	Similar	Somewhat
Wolf et al. (2008)	Reptile (Caretta caretta, Chelonia mydas, Lepidochelys kempii)	18.9–27.2	i-STAT (EC8+)	Centrifuge	Haematocrit	Lower	Somewhat
				Laboratory chemistry analyser	Na+	Similar	Somewhat
				Laboratory chemistry analyser	K+	Similar	Somewhat
				Laboratory chemistry analyser	Cl−	Lower	Somewhat
				Laboratory chemistry analyser	Glucose	Lower	Somewhat
				Laboratory chemistry analyser	BUN	Higher	Somewhat
Lieske et al. (2002)	Bird (Cerorhinca monocerata)	Not available	Accu-Chek Advantage	Reagent strips	Glucose	Similar	Yes
			Precision QID	Reagent strips	Glucose	Similar	Yes
			Glucometer Elite	Reagent strips	Glucose	Similar	Yes
			Sure Step	Reagent strips	Glucose	Similar	Yes
Burdick et al. (2012)	Mammal (Odocoileus virginianus)	Not available	IQ Prestige Smart	Laboratory/portable chemistry analyser	Glucose	Variable	No
			Prestige Smart	Laboratory/portable chemistry analyser	Glucose	Variable	No
Hopper and Cray (2007)	Mammal (Macaca fasicularis)	Not available	i-STAT (EC8+)	Centrifuge	Haematocrit	Lower	Somewhat
				Laboratory chemistry analyser	Na+	Higher	Somewhat
				Laboratory chemistry analyser	K+	Similar	Somewhat
				Laboratory chemistry analyser	Cl−	Higher	Somewhat
				Laboratory chemistry analyser	BUN	Higher	Somewhat
				Laboratory chemistry analyser	Glucose	Lower	Somewhat
				Laboratory chemistry analyser	Haemoglobin	Higher	Somewhat
				Laboratory chemistry analyser	TCO2	Higher	Yes
Larsen et al. (2002)	Mammal (Mirounga angustirostris)	Not available	i-STAT (6+)	Laboratory chemistry analyser	Na+	Lower	No
				Laboratory chemistry analyser	K+	Similar	Yes
				Laboratory chemistry analyser	Cl−	Higher	Somewhat
				Laboratory chemistry analyser	BUN	Similar	Yes
				Laboratory chemistry analyser	Glucose	Lower	Somewhat
				Centrifuge	Haematocrit	Similar	Yes

Na⁺, sodium; K⁺, potassium; Cl⁻, chloride; BUN, blood urea nitrogen; TCO₂, total carbon dioxide; pCO₂, partial pressure of carbon dioxide; HCO₃, bicarbonate; pO₂, oxygen partial pressure. For each species and analyte, the POC and standard (control) device are presented together with the relative comparison between the two. Where possible, the relevant body/experimental temperatures are presented. The distilled opinions presented by the authors of each study are also presented, but case-by-case caveats are not reported here. Many papers with ‘acceptable’ comparisons argue the need for corrective calculations or find these devices acceptable for relative rather than absolute measurement of an analyte.