Fig. 5.

Effect of extracellular Ca²⁺ on TCF-4 transcription in Ca²⁺-responsive and non-responsive CBS cells. Upper panel: TCF-4 transcription was measured using the dual luciferase reporter assay, and results were expressed as percent inhibition of CTAA in the presence of extracellular Ca²⁺ relative to control cells cultured in the absence of Ca²⁺. Lower panel: Cells were grown under Ca²⁺-free or Ca²⁺-containing conditions for 2 days. Lysates from these cells were probed for c-myc and cyclin D1 expression by Western blot analysis. (See Bhagavathula 2007 for details).