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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Arch Oral Biol. 2015 Nov 12;62:93–100. doi: 10.1016/j.archoralbio.2015.11.008

Figure 6.

Figure 6

(a) Light Microscope image of untreated bovine PTD. (b) Control, mineralized PTD untreated by antibodies. (c–e) Native tissue kept in PBS (not de-mineralized). (c) DPP monoclonal antibody. (d) Monoclonal anti-Chondroitin sulfate antibody. (e) Overlay of c and d. (f–h) Tissue soaked in 0.5 M EDTA in 20mM Tris buffer pH 8 (demineralized). (f) DPP monoclonal antibody (green). (g) Monoclonal anti-Chondroitin sulfate antibody (red). (h) Overlay of f and g, colocalization is indicated by yellow color.