Figure 2. Assessment of subunit-dependent effects of meprobamate on direct activation of GABAA receptors.
A, Representative traces demonstrating direct activation by meprobamate in α1β2γ2, α3β2γ2, α1β1γ2 and α1β2 receptors. B, C and D, summary data of meprobamate mediated direct-gating effects associated with varying α β and γ subunits, respectively. Direct activation capability was diminished in α3β2γ2 compared to the other configurations, whereas varying the β subunit or deleting the γ2 subunit had negligible effects. Each data point represents the mean ± S.E.M. of a minimum of 4 cells. *, P < 0.05; **, P < 0.01.