Fig 6. 5LO dimers cannot bind on nanodiscs as shown by the extensive stacking after staining by PTA.

(A) dimeric 5LO and ND incubated with Ca²⁺. Note, this preparation of nanodiscs was heterogenous and varied in the outer diameter of the discs, hence branches form (see boxes to the right). Circles show objects interpreted as unbound dimeric 5LO particles. (B) dimeric 5LO and ND incubated without Ca²⁺. Here, the preparation of nanodiscs did not vary in the outer diameter of the discs, hence no branches form. Instead, long stacks may lie on top of each other (top box) or lie parallel (middle and lower boxes). Dimeric 5LO are present (circles).