Table 1. 5LO initial velocity and product formation.
Initial velocities (column Vinit) and total 5-HPETE/5-HETE formation (column Final Product) were determined by UV spectroscopy at 235 nm, during 5 min incubations with different activating factors. Ratio 5LO:ND is 1.3:1. Data are mean±SEM (n = 3). LTA4 formation (column LT) in 10 min incubations with different activating factors was measured by LC-MS/MS. * Not detected. Data are mean±SEM(n = 2).
| Assay Components | Vinit μmol/mg per min | Final Product μmol/mg | LT μmol/mg |
|---|---|---|---|
| 5LO+AA | |||
| With Ca2+ | 0.20±0.02 | 0.70±0.08 | 0.69±0.04 |
| With out Ca2+ | 0.12±0.01 | 0.55±0.09 | 0.69±0.03 |
| 5LO+AA+ND | |||
| With Ca2+ | 1.87±0.12 | 3.60±0.10 | 3.29±0.25 |
| With out Ca2+ | 0.14±0.03 | 0.24±0.02 | 0.44±0.10 |
| 5LO+AA+PC | |||
| With Ca2+ | 1.35±0.06 | 3.32±0.13 | 2.41±0.05 |
| With out Ca2+ | 0.06±0.03 | 0.13±0.02 | n.d* |