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chKDM1AΔTower copurifies with a non-covalently bound FAD cofactor. chKDM1AΔTower was evaluated by UV–Vis and has absorption maxima at 380 and 455 nm (solid green line). The chimera was denatured by treatment with SDS, releasing the cofactor (dashed blue line). The spectrum of the denatured sample directly overlays with a FAD standard (solid black line).
